Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-12-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.569, 72.628, 124.183 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.690 - 2.430 |
| R-factor | 0.18817 |
| Rwork | 0.185 |
| R-free | 0.25006 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zxp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.506 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.700 | 2.540 |
| High resolution limit [Å] | 2.430 | 2.450 |
| Number of reflections | 14570 | |
| <I/σ(I)> | 14.43 | |
| Completeness [%] | 95.7 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100mM Sodium Citrate, 200mM Ammonium acetate, 20% Polyethylene glycol 4000 |
