5B6D
Crystal Structure of cytidine monophosphate hydroxymethylase MilA with CMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97912 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 109.601, 109.601, 113.432 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.459 - 1.650 |
R-factor | 0.1726 |
Rwork | 0.171 |
R-free | 0.19350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jnh |
RMSD bond length | 0.006 |
RMSD bond angle | 0.811 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
Rmerge | 0.132 | 0.094 | 0.885 |
Number of reflections | 91579 | ||
<I/σ(I)> | 4.4 | ||
Completeness [%] | 96.6 | 92.2 | 100 |
Redundancy | 6.7 | 6.3 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 289 | 0.1 M sodium cacodylate trihydrate(pH 6.5), 1.4 M sodium acetate trihydrate |