5B64
A novel binding mode of MAGUK GK domain revealed by DLG GK domain in complex with KIF13B MBS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | I 4 |
| Unit cell lengths | 116.279, 116.279, 72.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.771 - 2.700 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.069 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.800 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 13026 |
| <I/σ(I)> | 25.8 |
| Completeness [%] | 97.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1.0M Ammonium sulfate, 0.1M HEPES pH 7.0, 0.5%(w/v) Polyethylene glycol 8,000 |
