5B62

Crystal structure of N-terminal amidase with Asn-Glu-Ala peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97960
Spacegroup name	I 41 2 2
Unit cell lengths	133.796, 133.796, 118.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.041 - 3.042
R-factor	0.2107
Rwork	0.206
R-free	0.25380
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.041
High resolution limit [Å]	3.040
Number of reflections	10652
<I/σ(I)>	32.5
Completeness [%]	99.9
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M Mg acetate tetrahydrate, 0.2M Na cacodylate pH6.5, 20% (w/v) PEG 8000