5B61
Extra-superfolder GFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-10-24 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.301, 98.763, 263.804 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.382 - 3.115 |
R-factor | 0.1893 |
Rwork | 0.185 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.100 |
Number of reflections | 27784 |
<I/σ(I)> | 16.8 |
Completeness [%] | 97.8 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% Polyethylene glycol 4000, 0.1M tris pH 8.5, 0.2M Lithium sulfate |