5B5Z
Crystal structure of PtLCIB4 H88A mutant, a homolog of the limiting CO2-inducible protein LCIB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 48.079, 48.124, 59.922 |
Unit cell angles | 76.77, 87.96, 86.05 |
Refinement procedure
Resolution | 47.955 - 1.600 |
R-factor | 0.1806 |
Rwork | 0.179 |
R-free | 0.20720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b5y |
RMSD bond length | 0.007 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.960 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 68811 | |
<I/σ(I)> | 18.6 | |
Completeness [%] | 95.1 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 200 mM sodium acetate trihydrate, 100 mM Tris, 27% w/v PEG4000 |