5B5W

Crystal structure of MOB1-LATS1 NTR domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-26
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	78.747, 71.760, 57.855
Unit cell angles	90.00, 101.99, 90.00

Refinement procedure

Resolution	28.296 - 2.957
R-factor	0.2357
Rwork	0.234
R-free	0.27040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pi1
RMSD bond length	0.003
RMSD bond angle	0.745
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.957
Rmerge	0.074
Number of reflections	6660
<I/σ(I)>	21.4
Completeness [%]	94.1
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 4000