5B5S
Crystal structure of a carbohydrate esterase family 3 from Talaromyces cellulolyticus
Replaces: 3X0HExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.905, 70.905, 87.093 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.14788 |
| Rwork | 0.145 |
| R-free | 0.20305 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.096 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 35924 |
| <I/σ(I)> | 2.3 |
| Completeness [%] | 99.0 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 170mM ammonium sulfate, 28% PEG 4000, 5% glycerol, 0.5% n-octyl-beta-D-glucoside |
