5B5E

Crystal structure analysis of Photosystem II complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-18
Detector	RAYONIX MX-225
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	121.404, 228.224, 286.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.980 - 1.870
R-factor	0.17329
Rwork	0.171
R-free	0.21176
RMSD bond length	0.017
RMSD bond angle	2.383
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.000
High resolution limit [Å]	1.870
Rmerge	0.130
Number of reflections	649016
<I/σ(I)>	17.2
Completeness [%]	99.9
Redundancy	11.3
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.1	285	Mes,NaCl,CaCl2,MgSO4