5B59

Hen egg-white lysozyme modified with a keto-ABNO.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2015-09-30
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	79.030, 79.030, 36.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.18522
Rwork	0.182
R-free	0.24823
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lyz
RMSD bond length	0.016
RMSD bond angle	1.829
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	8266
<I/σ(I)>	16.3	9.4
Completeness [%]	99.7	100
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.8M NaCl, 0.05M AcOH