5B56

Crystal structure of HIV-1 VPR C-Terminal domain and DIBB-M-Importin-Alpha2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-11
Detector	RIGAKU SATURN A200
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	91.021, 81.555, 101.545
Unit cell angles	90.00, 97.78, 90.00

Refinement procedure

Resolution	31.520 - 2.300
R-factor	0.184
Rwork	0.182
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3knd
RMSD bond length	0.003
RMSD bond angle	0.724
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.1_1168)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.523	2.340
High resolution limit [Å]	2.298	2.300
Rmerge	0.056
Number of reflections	57284
<I/σ(I)>	24.7
Completeness [%]	87.0	51
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	50MM MES, 100MM AMMONIUM SULFATE, 10MM MGCL2, 20-23%(W/V) PEG8000, 5MM DTT