5B52

Crystal structure of the N-terminal domain of H-NS family protein TurB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-11-24
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	30.555, 143.350, 75.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.030 - 2.300
R-factor	0.24457
Rwork	0.242
R-free	0.29624
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.922
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	2.900	2.380
High resolution limit [Å]	2.300	2.800	2.300
Rmerge	0.048	0.976	0.632
Number of reflections	7892
<I/σ(I)>	57.2	2.7	4.1
Completeness [%]	99.6	100	100
Redundancy	13.9	13.1	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	ammonium acetate, BIS-TRIS propane
1	VAPOR DIFFUSION, HANGING DROP		293	ammonium acetate, BIS-TRIS propane