5B4Z
Crystal structure of heme binding protein HmuT H141A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-29 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.380, 73.380, 147.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.940 - 1.300 |
| R-factor | 0.15195 |
| Rwork | 0.151 |
| R-free | 0.16894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5az3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.940 | 1.340 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.058 | 0.551 |
| Number of reflections | 97636 | |
| <I/σ(I)> | 19.98 | 3.73 |
| Completeness [%] | 98.8 | 96.6 |
| Redundancy | 9.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.25 M Ammonium sulfate, 0.20 M Potassium thiocyanate, 0.20 M Ammonium tartrate, 5%(v/v) Polypropylene glycol, 0.025%(w/v) beta-Octyl glucoside |
