5B4Y

Crystal structure of the LA12 fragment of ApoER2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	31.162, 41.780, 55.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.280 - 1.900
R-factor	0.21197
Rwork	0.210
R-free	0.24667
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a7q
RMSD bond length	0.012
RMSD bond angle	1.674
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.086
Number of reflections	6043
<I/σ(I)>	9.5
Completeness [%]	99.9
Redundancy	5.2
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20%(wt./vol.) PEG monomethylether 2000, 100 mM Tris-Cl (pH 7.0)