5B4M
Crystal structure of an Fab against human influenza A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-07 |
| Detector | OXFORD RUBY CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.592, 90.940, 81.442 |
| Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
| Resolution | 81.380 - 2.400 |
| R-factor | 0.28785 |
| Rwork | 0.285 |
| R-free | 0.33796 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.561 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.380 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.445 |
| Number of reflections | 27507 | |
| <I/σ(I)> | 16.1 | 2.2 |
| Completeness [%] | 98.5 | 97.7 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | PEG 3350, NaCl, Bis-Tris |
