5B4D
Crystal structure of H10N mutant of LpxH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.564, 87.888, 98.358 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.180 - 1.750 |
R-factor | 0.17949 |
Rwork | 0.178 |
R-free | 0.20150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b49 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.464 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 59677 | |
<I/σ(I)> | 18.4 | 2.13 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M Proline, 0.1M HEPES pH 7.0, 10.0% PEG 3350 |