5B40
The nucleosome structure containing H2B-K120 and H4-K31 monoubiquitinations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-17 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.1 |
Spacegroup name | P 32 |
Unit cell lengths | 100.419, 100.419, 186.025 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.391 - 3.330 |
R-factor | 0.2052 |
Rwork | 0.201 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3av1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.496 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.460 |
High resolution limit [Å] | 3.330 | 6.920 | 3.330 |
Rmerge | 0.070 | 0.052 | 0.320 |
Number of reflections | 30012 | ||
<I/σ(I)> | 11.4 | ||
Completeness [%] | 97.7 | 99.6 | 92.8 |
Redundancy | 6.2 | 9.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 90mM Tris-HCl (pH 7.8), 3.6% PGA-LM, 25.2% PEG 400, 2-6% Pentaerythritol ethoxylate (3/4 EO/OH) |