5B3N
The crystal structure of anti-H4K20me1_scFv, 15F11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 130.825, 130.825, 130.825 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.608 - 1.940 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ghw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.085 |
| Data reduction software | HKL-2000 (706c) |
| Data scaling software | HKL-2000 (706c) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 4.180 | 1.940 |
| Rmerge | 0.099 | 0.073 | 0.403 |
| Number of reflections | 29161 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 99.9 | 99.8 | 99.4 |
| Redundancy | 28 | 54.9 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | Sodium acetate, poly-gamma-glutamic acid polymer, and PEG2000 |
