5B3H
The crystal structure of the JACKDAW/IDD10 bound to the heterodimeric SHR-SCR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-04 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.668, 203.389, 88.451 |
| Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
| Resolution | 39.438 - 2.700 |
| R-factor | 0.2096 |
| Rwork | 0.208 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b3g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.968 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 54886 | |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | tris-HCl (pH 8.0) buffer, 8% w/v PEG 3350 |
