5B3G
The crystal structure of the heterodimer of SHORT-ROOT and SCARECROW GRAS domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-26 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.700, 84.428, 89.797 |
| Unit cell angles | 90.00, 92.66, 90.00 |
Refinement procedure
| Resolution | 33.101 - 2.000 |
| R-factor | 0.1859 |
| Rwork | 0.183 |
| R-free | 0.22960 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.978 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 56799 | |
| <I/σ(I)> | 28.1 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | phosphate buffer (pH 7.0), polyethylene glycol (PEG) 3350 |
