5B3F
Crystal structure of phosphoribulokinase from Methanospirillum hungatei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.414, 93.822, 99.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.020 - 2.500 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.27900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.020 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.274 | |
| Number of reflections | 24121 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 92.2 | 90 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.8 | 293 | PEG4000, lithium sulfate |
