5B38
KIR3DL1*005 in complex with HLA-B*57:01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.557, 61.271, 66.365 |
| Unit cell angles | 95.20, 100.25, 108.22 |
Refinement procedure
| Resolution | 35.496 - 2.300 |
| R-factor | 0.1984 |
| Rwork | 0.196 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vh8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.702 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 61910 | |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 92.9 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 14 to 18% PEG 3350, 2% tacsimate pH 5.0, 0.1 M tri-sodium citrate pH 5.6 |
