5B36
Crystal Structure of the O-Phosphoserine Sulfhydrylase from Aeropyrum pernix Complexed with Cysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.181, 74.181, 278.994 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.150 |
R-factor | 0.18335 |
Rwork | 0.180 |
R-free | 0.24598 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | CysO free form (PDB ID 3VSA) |
RMSD bond length | 0.024 |
RMSD bond angle | 2.091 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 2.180 |
High resolution limit [Å] | 2.150 | 2.140 |
Number of reflections | 43621 | |
<I/σ(I)> | 17 | |
Completeness [%] | 92.0 | |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.1 M HEPES sodium pH 7.5, 27% 2-propanol, 10% PEG4000, 12 mM TCEP-HCl, the crystal was soaked with the reservoir solution containing 6% MPD as a cryoprotectant, 20 mM L-cysteine, and 12 mM TCEP-HCl |