5B33

The crystal structure of the H2AZ nucleosome with H3.3.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-10
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	104.799, 109.872, 181.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.005 - 2.925
R-factor	0.2076
Rwork	0.205
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3av2
RMSD bond length	0.011
RMSD bond angle	1.202
Data scaling software	HKL-2000 (0.98.704k)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.020
High resolution limit [Å]	2.920	6.290	2.920
Rmerge	0.070	0.048	0.474
Total number of observations	311643
Number of reflections	45941
<I/σ(I)>	14.1
Completeness [%]	99.7	97.3	100
Redundancy	6.8	6.2	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride