5B2I
Human nucleosome containing CpG unmethylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.499, 109.047, 177.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.820 - 3.000 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cv5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.576 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.820 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.145 | |
| Number of reflections | 41785 | |
| <I/σ(I)> | 4.87 | |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 4.77 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | potassium cacodylate, manganese(II) chloride, potassium chloride |
