5B2D
Crystal structure of Mumps virus hemagglutinin-neuraminidase bound to 3-sialyllactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 136.875, 136.875, 177.274 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 118.537 - 2.177 |
| R-factor | 0.1783 |
| Rwork | 0.176 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e8v |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.109 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 177.274 | 2.184 |
| High resolution limit [Å] | 2.177 | 2.177 |
| Rmerge | 1.083 | |
| Number of reflections | 98249 | |
| <I/σ(I)> | 10.9 | 2 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 10 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium acetate, ammonium sulfate, glycerol, 3-sialyllactose |
