5B2C

Crystal structure of Mumps virus hemagglutinin-neuraminidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-29
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1
Spacegroup name	P 61
Unit cell lengths	137.523, 137.523, 178.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	119.098 - 2.238
R-factor	0.2036
Rwork	0.202
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e8v
RMSD bond length	0.004
RMSD bond angle	0.888
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.100	2.245
High resolution limit [Å]	2.238	2.238
Rmerge		1.709
Number of reflections	91923
<I/σ(I)>	14.3	2.1
Completeness [%]	100.0	99.9
Redundancy	10.2	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium acetate, ammonium sulfate, glycerol