5B2A
Crystal structure of a mutant of OspA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.370, 53.658, 65.756 |
Unit cell angles | 90.00, 100.35, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
R-factor | 0.20333 |
Rwork | 0.201 |
R-free | 0.25013 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.057 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 27889 | |
<I/σ(I)> | 27.7 | |
Completeness [%] | 97.2 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 40% PEG 400, 0.1M Tris pH 9.0 |