5B24
The crystal structure of the nucleosome containing cyclobutane pyrimidine dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-11 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.848, 108.868, 174.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.640 - 3.600 |
R-factor | 0.2069 |
Rwork | 0.205 |
R-free | 0.24950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3afa |
RMSD bond length | 0.004 |
RMSD bond angle | 0.746 |
Data scaling software | HKL-2000 (0.98.701) |
Phasing software | PHASER (2.5.1) |
Refinement software | PHENIX (1.10.1-2155-000) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 7.750 | 3.600 |
Rmerge | 0.111 | 0.032 | 0.475 |
Total number of observations | 94396 | ||
Number of reflections | 22837 | ||
<I/σ(I)> | 7.3 | ||
Completeness [%] | 96.0 | 91.3 | 97.6 |
Redundancy | 4.1 | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |