5B1Q
Human herpesvirus 6B tegument protein U14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.330, 211.530, 51.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.680 - 1.850 |
| R-factor | 0.1809 |
| Rwork | 0.180 |
| R-free | 0.23280 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.002 |
| Data scaling software | XDS (20141118) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.680 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.087 | 0.575 |
| Number of reflections | 93926 | |
| <I/σ(I)> | 18.04 | 3.39 |
| Completeness [%] | 99.8 | 99.13 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277.15 | 0.2M Potassium sodium tartrate tetrahydrate, 16-18% w/v Polyethylene glycol 3350 |
