5B1N
DHp domain structure of EnvZ from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-15 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 62 |
| Unit cell lengths | 38.510, 38.510, 84.508 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.255 - 1.330 |
| R-factor | 0.1784 |
| Rwork | 0.177 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zrx |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.623 |
| Data reduction software | SCALA |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER (2.5.5) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.258 | 42.258 | 1.400 |
| High resolution limit [Å] | 1.200 | 4.210 | 1.330 |
| Rmerge | 0.052 | 0.030 | 0.418 |
| Rmeas | 0.057 | ||
| Rpim | 0.022 | 0.014 | 0.176 |
| Total number of observations | 104426 | 1865 | 15234 |
| Number of reflections | 15966 | ||
| <I/σ(I)> | 18.1 | 28.5 | 4.5 |
| Completeness [%] | 97.9 | 65.3 | 100 |
| Redundancy | 6.5 | 5.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 1.1-1.3 M sodium/potassium phosphate |
