5B1M

The mouse nucleosome structure containing H3.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.100
Spacegroup name	P 21 21 21
Unit cell lengths	98.715, 107.663, 168.342
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.593 - 2.340
R-factor	0.2138
Rwork	0.211
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3afa
RMSD bond length	0.009
RMSD bond angle	1.068
Data scaling software	HKL-2000 (705b)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.420
High resolution limit [Å]	2.340	5.040	2.340
Rmerge	0.089	0.056	0.489
Rmeas	0.095	0.059	0.563
Rpim	0.033	0.019	0.271
Total number of observations	519592
Number of reflections	74131
<I/σ(I)>	9.3
Completeness [%]	96.0	92.9	94.4
Redundancy	7	8.9	3.6
CC(1/2)		0.997	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride