5B1G

Crystal structure of hen egg-white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	300
Detector technology	CCD
Collection date	2014-11-25
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 43 21 2
Unit cell lengths	80.300, 80.300, 38.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.390 - 2.300
R-factor	0.1825
Rwork	0.181
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wul
RMSD bond length	0.009
RMSD bond angle	1.162
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	5983
<I/σ(I)>	9.8	7.6
Completeness [%]	100.0	100
Redundancy	502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	NaCl