5B1E

Crystal structure of proteinase K from Engyodontium album

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	300
Detector technology	CCD
Collection date	2014-11-25
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 43 21 2
Unit cell lengths	68.900, 68.900, 109.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.659 - 2.300
R-factor	0.1429
Rwork	0.141
R-free	0.18120
RMSD bond length	0.008
RMSD bond angle	1.055
Data scaling software	CrystFEL
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Number of reflections	12263
<I/σ(I)>	12.4
Completeness [%]	100.0
Redundancy	402

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		291	NaNO3, CaCl2, MES