5B1A
Bovine heart cytochrome c oxidase in the fully oxidized state at 1.5 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 50 |
| Detector technology | CCD |
| Collection date | 2010-04-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 181.938, 204.400, 177.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.500 |
| R-factor | 0.14998 |
| Rwork | 0.149 |
| R-free | 0.17190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.040 |
| RMSD bond angle | 3.569 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.107 | |
| Number of reflections | 1041861 | |
| <I/σ(I)> | 32.7 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.9 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.8 | 277 | PEG 4000, Sodium phosphate |
