5B0Z
The crystal structure of the nucleosome containing H3.2, at 1.98 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.685, 108.204, 168.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.942 - 1.987 |
| R-factor | 0.2133 |
| Rwork | 0.211 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cv5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.479 |
| Data reduction software | HKL-2000 (705b) |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 4.260 | 1.980 |
| Rmerge | 0.073 | 0.048 | 0.481 |
| Rmeas | 0.077 | 0.050 | 0.531 |
| Rpim | 0.025 | 0.015 | 0.217 |
| Total number of observations | 957221 | ||
| Number of reflections | 119479 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 96.3 | 99 | 90.6 |
| Redundancy | 8 | 10.7 | 5.3 |
| CC(1/2) | 0.999 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |
