5B0Q
beta-1,2-Mannobiose phosphorylase from Listeria innocua - mannose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 146.212, 146.212, 105.841 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.860 - 2.300 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.21719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkd |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.102 | 0.581 |
| Number of reflections | 58114 | |
| <I/σ(I)> | 22.7 | 3.5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8.7 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 1.8 M (NH4)2SO4, 10 mM CoCl2, 0.1 M MES-NaOH |
