5B0H

CRYSTAL STRUCTURE OF HUMAN LEUKOCYTE CELL-DERIVED CHEMOTAXIN 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-10-30
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.415, 63.514, 63.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.070 - 1.940
R-factor	0.179
Rwork	0.177
R-free	0.22300
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.745
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.940	1.940
Number of reflections	18525
<I/σ(I)>	51.9
Completeness [%]	99.7	97.2
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% PEG8000, 0.2M AMMONIUM SULFATE, 0.1M HEPES