5AZZ
Crystal structure of seleno-insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9780 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 77.994, 77.994, 77.994 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.000 - 1.450 |
| R-factor | 0.1696 |
| Rwork | 0.168 |
| R-free | 0.19160 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.063 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.124 | 0.933 |
| Number of reflections | 27239 | |
| <I/σ(I)> | 11.4 | 2.6 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 11.4 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | Sodium chloride, ethylene imine polymer, citrate |
