5AZY

Crystal structure of p24delta1 GOLD domain (Native 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	CCD
Collection date	2011-11-16
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	66.798, 77.354, 41.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.471 - 1.800
R-factor	0.2354
Rwork	0.233
R-free	0.28050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5azx
RMSD bond length	0.006
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge		0.563
Number of reflections	20574
<I/σ(I)>	56.7	4.6
Completeness [%]	98.0	100
Redundancy	7.1	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8 M ammonium citrate (pH 7.0)