5AZX
Crystal structure of p24delta1 GOLD domain (native 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-11-07 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.232, 74.392, 79.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.720 - 1.580 |
R-factor | 0.2306 |
Rwork | 0.230 |
R-free | 0.24930 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.862 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.459 | |
Number of reflections | 56232 | |
<I/σ(I)> | 78.7 | 6.3 |
Completeness [%] | 99.4 | 100 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M sodium citrate (pH 5.5) and 2.0 M ammonium sulfate |