5AZX

Crystal structure of p24delta1 GOLD domain (native 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-11-07
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	68.232, 74.392, 79.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.720 - 1.580
R-factor	0.2306
Rwork	0.230
R-free	0.24930
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.610
High resolution limit [Å]	1.580	1.580
Rmerge		0.459
Number of reflections	56232
<I/σ(I)>	78.7	6.3
Completeness [%]	99.4	100
Redundancy	7.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M sodium citrate (pH 5.5) and 2.0 M ammonium sulfate