5AZT
Ternary complex of hPPARalpha ligand binding domain, 17-oxoDHA and a SRC1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 59.460, 59.460, 317.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.000 - 3.450 |
R-factor | 0.25537 |
Rwork | 0.253 |
R-free | 0.30763 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.257 |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.610 |
High resolution limit [Å] | 3.450 |
Number of reflections | 8185 |
<I/σ(I)> | 15.6 |
Completeness [%] | 98.5 |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 0.1M HEPES, 35% PEG3350 |