5AZP
Crystal structure of a membrane protein from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-23 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | I 4 |
| Unit cell lengths | 257.515, 257.515, 81.825 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.690 |
| R-factor | 0.16109 |
| Rwork | 0.160 |
| R-free | 0.17974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5azo |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.973 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.690 |
| Number of reflections | 297648 |
| <I/σ(I)> | 22.9 |
| Completeness [%] | 99.5 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 4000, sodium acetate, sodium formate |
