5AZH

Crystal structure of LGG-2 fused with an EEEWEEL peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	CCD
Collection date	2015-09-28
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	35.532, 55.193, 72.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.940 - 2.300
R-factor	0.23826
Rwork	0.236
R-free	0.28827
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.245
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.940	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.104	0.859
Number of reflections	7374
<I/σ(I)>	29.3
Completeness [%]	98.3	85.9
Redundancy	18.4	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium chloride, magnesium chloride, HEPES