5AZH
Crystal structure of LGG-2 fused with an EEEWEEL peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-09-28 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.532, 55.193, 72.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.940 - 2.300 |
| R-factor | 0.23826 |
| Rwork | 0.236 |
| R-free | 0.28827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.245 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.940 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.104 | 0.859 |
| Number of reflections | 7374 | |
| <I/σ(I)> | 29.3 | |
| Completeness [%] | 98.3 | 85.9 |
| Redundancy | 18.4 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium chloride, magnesium chloride, HEPES |
