5AZE
Fab fragment of calcium-dependent antigen binding antibody, 6RL#9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-11-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.470, 79.860, 116.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.2145 |
| Rwork | 0.211 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ZA6 for VH CL and CH1 regions 2A9M for VL region |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.212 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.350 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.113 | 0.628 |
| Number of reflections | 22199 | |
| <I/σ(I)> | 14.8 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.12 | 7.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20.0% PEG 4000, 0.1M HEPES |
