5AZ7
Crystal structure of MBP-Tom20 fusion protein with a 4-residue spacer in the connector helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.109, 113.908, 125.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.220 - 1.960 |
| R-factor | 0.19861 |
| Rwork | 0.196 |
| R-free | 0.24196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf 2v1t |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.960 |
| Number of reflections | 30663 |
| <I/σ(I)> | 14.6 |
| Completeness [%] | 93.1 |
| Redundancy | 6.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 50% PEG 400, 0.1M Phosphate citrate (pH 4.4) |
