5AZ4

Crystal structure of a 79KDa fragment of FlgE, the hook protein from Campylobacter jejuni

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-20
Detector	RAYONIX MX225HE
Wavelength(s)	0.9791, 0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	75.457, 173.541, 147.086
Unit cell angles	90.00, 102.66, 90.00

Refinement procedure

Resolution	24.956 - 2.450
R-factor	0.204
Rwork	0.202
R-free	0.22870
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.844
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((PHENIX.AUTOSOL: 1.7.1))
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	24.960
High resolution limit [Å]	2.450
Number of reflections	133379
<I/σ(I)>	18.34
Completeness [%]	98.4
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	288	1.2 M K2HPO4, 50 mM NaCl, 5 mM Tl2SO4, 12% DMSO, 50 mM MES pH 6.0
1	VAPOR DIFFUSION, HANGING DROP	6	288	1.2 M K2HPO4, 50 mM NaCl, 5 mM Tl2SO4, 12% DMSO, 50 mM MES pH 6.0