5AZ0
Crystal structure of aldo-keto reductase (AKR2E5) of the silkworm, Bombyx mori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-20 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 112.180, 112.180, 147.628 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.000 - 2.200 |
R-factor | 0.15905 |
Rwork | 0.157 |
R-free | 0.20368 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.909 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 28491 |
<I/σ(I)> | 35.6 |
Completeness [%] | 99.8 |
Redundancy | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 4M ammonium acetate |