5AYW

Structure of a membrane complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 41 21 2
Unit cell lengths	116.428, 116.428, 434.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.568 - 3.555
R-factor	0.2741
Rwork	0.271
R-free	0.31410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.669
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.550
Number of reflections	37045
<I/σ(I)>	20
Completeness [%]	99.5
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	Tris-HCl, NaCl, PEG 400