5AYU
Crystal structure of HyHEL-10 Fv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-01-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.822, 65.822, 96.321 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.19344 |
| Rwork | 0.191 |
| R-free | 0.23359 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c08 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.441 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | 0.296 |
| Number of reflections | 22939 | |
| <I/σ(I)> | 29.8 | 9.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.9 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 293 | 0.1M Tris-HCl, 1.5M ammonium sulfate, 12% glycerol, pH 9.2 |
